UV/Vis Single Beam Spectrophotometer - Vision 1000

Microprocessor UV/ VIS Spectrophotometer

Neptune Vision-1000 & Vision-2000 Series is an advance Single & double beam design consisting of two Models.

Double beam UV-VIS Spectrophotometer with more accuracy and flexible requirements. The two detectors are used to measure sample and reference respectively and simultaneously for optimizing measurement accuracy. They are suitable for pharmaceutical, Bio- Chemical & Clinical lab applications as well as routine applications such as quantitative analysis, Kinetics, Wavelength Scan, Multiple components and DNA/ Protein, PC windows application software make these instruments versatile. All instruments provide excellent performance for measurements.

Technical SPECIFICATIONS:-

Model Vision- 1000(Single Beam)

Optical System Single-Beam(1200 lines/mm Grating)

Wavelength Range 190- 1100nm

Wavelength Accuracy +_1nm

Wavelength Repeatability 0.5nm

Stray Light _<0.3 %T

Mode -

Scanning Speed Fast/ Medium/ Low

Band Width 2nm

Photometric Accuracy +_0.5% T

Photometric Repeatability +_0.3% T

Photometric Range Absorbance:0.3 to 3.0A/ Transmittance : 0 to 200% T

Stability 0.001 A/h @ 500nm

Light Source Tungsten Halogen Lamp & Deuterium Lamp

Power Supply AC 100 V/ 220 V, 50Hz

Detector Silicon Photodiode

Scan Speed -

Wavelength Change Appx. 3800nm/ minute

Scan -

Baseline Stability 0.001 A/ h

Noise Level 0.002Abs

Data Output Port USB Port & Parallel Port (Printer)

Weight 16.950kg

Display Graphic LCD (128*64 Dots)

Overall Dimensions(L*W*H) 420 X 280 X 180mm

Standard Configuration:-

Main Instrument 1 Unit

Operation Manual 1 Pc

Glass Cell 10nm 4 Pc

Quartz Cell 10nm 2 Pc

Power Cable 1 Pc

Fuse (3A) 2 Pc

Software CD 1 No.

Software Key 1 No.

Software Manual 1 Pc

USB Cable 1 No.

Dust Cover 1 No.

UV CONTROL SOFTWARE:-

The Neptune windows based PC application software UV/Vis analyst takes the best features of the stand alone version plus more powerful data processing, expanded data collecting and storage capability. It comes standard with Neptune PC models and is optional to stand alone models.

The PC application software offers:-

1) Photometric Mode

2) Quantitative test (standard curve)

3) Wavelength Scan

4) Kinetics

5) DNA/Protein

6) Multi-Wavelength

7) System Utility

Photometric Mode (Basic Mode)

To measure the Absorbance and Transmittance.

Quantitative test (standard curve)

Use up to 20 standards samples may be used to establish standard curve. Four methods for fitting a Curve through the calibration points:-

1) Linear fit

2) Linear through zero.

3) Square fit.

4) Cubic fit.

Wavelength Scan

Automatically record peaks & Valleys. The quantity of channels is unlimited. You can simultaneously store as many as desired. Post run manipulation and processing includes:

1) The wavelength scan intervals are 0.1, 0.2, 0.5, 1, 2, 5nm.

2) Hi, Medium and Low scan speeds are available. They vary from 100 to 3600 nm/ min.

3) Wavelengths are scanned from high to low so that the instrument waits at high WL. And it minimizes the degradation of UV sensitive samples.

Kinetics (Abs vs. Time)

The kinetic mode may be for time course scanning or reaction rate calculations. Abs vs time graphs are displayd on the screen in real time. Waiting time measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2, 5, 10, 30 seconds and 1min. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algression algorithm before multiplying by the entered factor.

DNA/Protein Test

Concentration and DNA purity are quickly and easily calculated:

Absorbance ratios 260 nm/ 280 nm with optional subtracted absorbance at 320 nm.

DNA concentration= 62.9 x 260 – 36.0 x A280

Protein Concentration= 1552 x A260 – 757.3 X A280

Other wavelength and factors may be entered.

Multi-Wavelength

Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required to run multiple samples automatically.